Differentiation of Streptococcus pneumoniae from other viridans streptococci depends on demonstrating optochin susceptibility, bile solubility, reaction with a specific DNA probe, or detection of species-specific capsular polysaccharides. Most clinical microbiology laboratories today depend on the optochin susceptibility test. Catalase test is used to differentiate staphylococci from streptococci. Pneumococcus can be differentiated from streptococcus by Bile solubility … INTRODUCTION. Related Articles . Streptococcus pyogenes Introduction. DIFFERENTIAL DIAGNOSTIC TEST. This test is used to differentiate Streptococcus pneumoniae, which is soluble in bile and bile salts, from alpha- hemolytic Streptococcus which are insoluble.. RAPID METHOD FOR DEMONSTRATING BILE SOLUBILITY OF DIPLOCOCCUS PNEUMONIAE. The Bile Solubility Test is one of the most important tests which is commonly used to differentiate the Streptococcus pneumoniae, which is positive for bile solubility test, from the other alpha-hemolytic streptococci like Streptococcus pyogenes, which is Bile Solubility test Negative. It is a low cost, rapid … P-disk or Bile solubility Bacitracin disk PYR Growth on bile esculin Growth in NaCl. Streptococcus pyogenes is the only member of Lancefield group A Also call Group A Streptococcus (GAS) Streptococcus pyogenes Reservoir. Differentiation of Streptococcus pneumoniae from other Streptococcus mitis group streptococci (SMGS) remains challenging despite the introduction of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Bile-esculin test is widely used to differentiate Enterococci and Non-enterococcus group D streptococci, which are bile tolerant and can hydrolyze esculin to esculetin, from non-group D viridans group streptococci, which grow poorly on bile. HAWN CV, BEEBE E. PMCID: PMC315678 PMID: 14329473 [PubMed - indexed for MEDLINE] MeSH Terms. Finally, viridans streptococci also lack the polysaccharide-based capsule typical of Streptococcus pneumoniae. Bile Solubility Test- Principle, Procedure, Result Interpretation, Examples and Limitation. Bacteriological Techniques* Bile* Bile Acids and Salts* Culture Media* Escherichia coli* Haemophilus influenzae* Metabolism* Neisseria* Research* Salts* Solubility* Staphylococcus* Streptococcus* Streptococcus … Some M types of streptococcus pyogenes produce a lipoproteinase which results in opacity when applied to agar gel. Streptococcus pneumoniae is a major cause of community-acquired pneumonia (CAP). Strep pneumoniae is bile soluble, meaning that the bile salts will promote the lysis of the bacteria; whereas viridans streptococci are bile insoluble, or bile resistant. Principle.